Stem cells have been drawing increasing interest in basic and translational research that aims to understand stem cell biology and generate new therapies for various disorders. Many stem cells can be cultured in 2D relatively easily using tissue culture plastic. However, many of these cultures do not represent the natural conditions of stem cells in the body. In the body, microenvironments include numerous supporting cells and molecules. Therefore, researchers and clinicians have sought ideal stem cell preparations for basic research and clinical applications. This book brings together recent research on cultures of various stem cells to increase the basic understanding of stem cell culture techniques and also to highlight stem cell preparations for possible novel therapeutic applications. This book includes experiments of stem cell and regenerative subject. The purpose of this practical book is to educate interested students about the detailed methodology of stem cell experiments. Along with methodology this book covers an aim, objective and schematic representation of related experiment. Book starts from the basic culturing of cells and furthermore explains detailing of isolation of stem cells, their characterization, introduction or basic handling of induced pluripotent stem cells (iPSCs). It provides compositions of different cell culture media as per the cell requirement in the tabular format. Diagrammatic format of any practical eases the process of learning. Hence, diagrammatic representation has been provided for each experiment. We hope this book will inspire students to perform stem cell experiments and invent new ideas in the field of Stem Cell Culture. This Book is an invaluable resource for researchers, teachers, and students who are interested in or working with stem cell culturing.