This analysis can be used to define the patterns which can infer the molecular structure, experimental analysis of molecular structure and function, and further understanding on development of smaller molecules for further biological research. Structure probing analysis can be done through many different methods, which include chemical probing, hydroxyl radical probing, SHAPE, nucleotide analogue interference mapping (NAIM), and in-line probing. While methods for each type of probing differ in steps, the probing of secondary structure involves certain steps in order to determine the structure. The first step involved is submitting the structural RNA to the probe of interest and incubating over a certain amount of time to allow the reaction to occur. The RNA is then transcribed using reverse transcriptase PCR, where this results in different lengths of bands due to the modification of the RNA at specific sites, which causes the reverse transcriptase to fall off. These bands are then run on a gel with sequencing data determined from RNA sequencing. RNA chemical probing can involve many different chemicals which serve to modify specific bases at certain sites to show certain locations available for specific modification type. This textbook covers essentially every aspect of the field of molecular biotechnology, which is constantly changing and adapting in light of new advances.